Determination of di-2-ethylhexyl phthalate levels in human blood plasma and cryoprecipitates.
نویسنده
چکیده
While investigating the possible occurrence of neutral plasmalogens in human blood, we isolated a substance which had the same chromatographic characteristics on thin-layer chromatoplates as those of alkyl diglycerides. This substance was identified (1) as di-2-ethylhexyl phthalate (DEHP), and we were able to demonstrate (2) that this plastifier originated from the plastic transfusion packs which had contained the blood and that its concentration increased with the time of storage up to a level of about 11.5 mg/100 ml of plasma after 21 days. These observations were confirmed by Jaeger and Rubin (3), who have also shown that DEPH accumulates in liver cells during the perfusion of rat liver. These authors also found that liver, spleen, lung, and abdominal fat in two patients who had received blood transfusions contained significant quantities of DEHP. In the present report, we describe a direct method of determination of DEHP by gasliquid chromatography. With this method, we have been able to demonstrate that the leaching out of DEHP from plastic transufsion packs was correlated with the plasma concentration of triglycerides. Also the DEHP levels in cryoprecipitates, which are
منابع مشابه
Investigation of Di-ethylhexyl Phthalate Migration by Applying Magnetic Solid Phase Extraction Method Followed by GC-FID Determination
Background and Objectives: The addition of plasticizers (mainly phthalates and adipates) makes plastic flexible and popular for packaging, storing and preserving food, water, and so on. The most widely used plasticizer in food contact applications is di-ethylhexyl phthalate (DEHP) though being suspected to have carcinogenic and estrogenic properties. The aim of this study is application of magn...
متن کاملSelective Extraction and Determination of Di(2-ethylhexyl) Phthalate in Aqueous Solution by HPLC Coupled with Molecularly Imprinted Solid-phase Extraction
Surface Molecularly Imprinted Polymer (SMIP) for selective adsorption of di(2-ethylhexyl) phthalate(DEHP) was prepared on the surface of silica gel which was modified by aminopropyltriethoxysilane and acryloyl chloride in a two-step method. The prepared SMIP was used to prepare Molecularly Imprinted Solid-Phase Extraction (MISPE) column for selective extraction of DEHP from aqueous solution...
متن کاملValidation and application of an HPLC method for determination of di (2-ethylhexyl) phthalate and mono (2-ethylhexyl) phthalate in liver samples.
A reversed-phase gradient elution, UV detection method is developed for the simultaneous determination of mono (2-ethylhexyl) phthalate [MEHP] and di (2-ethylhexyl) phthalate [DEHP] in tissue samples. The method is validated with respect to extraction recovery, inter and intra-day precision, linearity of response, detect ability, and specificity. The validated method has been successfully appli...
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In order to impart flexibility, plastic medical devices incorporate liquid plasticizers into their structure. Data from several laboratories, including ours, have shown that these compounds leach from blood bags and tubing during collection of blood, storage of various blood components and during kidney dialysis and cell and plasma apheresis procedures. After the plasticizer di(2-ethylhexyl) ph...
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ورودعنوان ژورنال:
- Environmental Health Perspectives
دوره 3 شماره
صفحات -
تاریخ انتشار 1973